Products

We specialize in modified oligos, fluorescent probes, long oligos and large scale oligo synthesis. The more modifications that are present in an oligo, and the longer the sequence, the more demanding the synthesis and purification.

Most of the oligos we make contain modifications, and many contain several different modifications.

This is a list of our most frequently requested modifications.

• FAM (fluorescein), HEX, JOE, ROX, TAMRA, TET and Texas Red®
• Fluorescein dT and Tamra dT
• Cyanine dyes

FAM, JOE, TAMRA and ROX are the “big four” dyes that have dominated DNA sequencing. The Cyanine dyes have found widespread use in DNA and RNA labelling.

• FLUORESCENCE QUENCHERS
• FRET fluorescence quenchers (including BHQs)
• Dabcyl and other collisional quenchers

Fluorescence quenchers absorb energy from a fluorophore (fluorescent dye), so that the fluorophore does not emit light when excited by light at a different wavelength. Quenchers operate via two distinct mechanisms: FRET quenching and collisional quenching.

• Deoxyinosine
• Deoxyuridine
• Amino dU
• 2-Aminopurine
• 5-Bromodeoxycytidine
• 5-Bromodeoxyuridine
• Aminohexyl (aminolink)
• Phosphate
• Thiol
• Hexaethylene glycol
• Thiophosphate
• 5-Iododeoxyuridine
• 5-Methyldeoxycytidine

Many other modifications are available; please enquire for further details.

It is a relatively new method for labelling oligos and ligating oligos. These modifications prepare oligos for click reactions.

• Azido dU
• Octadiynyl dU
• OTHER MODIFICATIONS
• Digoxigenin
• Biotin
• Octanediol

Many other modifications are available; please enquire for further details.

• Phosphorothioate DNA
• 2′-O-methyl RNA
• RNA

Very many other 3′-, 5′- and internal modifications are possible.

We can synthesize oligos on the 30 μmol, 50 μmol, 100 μmol and 200 μmol scales. But if you are not sure, or if you need a specific amount of oligo, contact us and we will advise you on the best synthesis scale. Because we treat each oligo individually, and are sensitive to the particular chemistry of the oligo and modifications, we obtain the best possible quality and yields.

We usually purify oligos by HPLC. Where extra purity is required, we suggest Double HPLC purification.

We take quality control very seriously. We analyze oligos by HPLC, and optionally by mass spectrometry.

We supply a data sheet with each oligo, outlining details on the oligo sequence, physical properties (molecular weight, predicted melting temperature, extinction coefficient etc.) and quality control data (concentration, and total amount of oligo sent).

We supply oligos in solution in screw-top vials. The concentration (in OD mL-1, mol dm-3 and μg mL-1) is included on the attached data sheet. Alternatively, we can adjust the concentration to a value you specify.

We can also supply oligos as freeze-dried solids, which we recommend for long-term storage.

We ship our oligos via Fedex or DHL.